山东大学研究生导师介绍------申玉龙
申玉龙, published by www.lifesci.sdu.edu.cn print or close this page
prof. yulong shen (ph. d)
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i graduated from lanzhou university in 1986 and obtained a ph.d in plant cell and molecular biology in the university of birmingham, uk, in 1996. from 1997-1998, i worked as a sta (science and technology of japan) postdoctoral fellow in the institute of fruit tree science , and from 1999-2003, as a researcher in the institute of advanced industrial science and technology (aist), japan . i became a full professor of shandong university in november, 2003.
research interests
extremophiles are microorganisms that live in extreme environments, including environments with high temperature, low ph, high ph, and containing high concentration of salts etc. archaea, the most abundant extremophiles, possess information processing systems similar to that of euryarchaeota but in much simplified forms. therefore the study of archaea will help us understand the origin and nature of life, and to reveal the mechanism of dna replication, repair and recombination in euryarchaeota, which has become complicated during evolution. extremophiles can also provide us with more stable catalysts for industrial application.
the research areas in our lab include:
1. microbial resources from extreme environments, such as hot spring and deep see vent;
2. biochemistry of archeal hyperthermo-stable carbohysrate metabolizing enzymes and their application;
3. the molecular mechanism of dna replication, repair, and recombination in archaea.
(1) mre11/rad50 mediated dna recombination in sulfolobus;
(2) dna replication in nanoarchaeum equitans , a recently found archaeon representing a new phylum;
(3) recombinational repair of dna in sulfolobus.
4. crystal structure analysis of thermostable enzymes in carbohydrate degradation and dna
replication.
research grants
the research in our group is supported by three major grants: a sub-project of a national program for basic research of china (973) (2004-2008), two projects from the national science foundation of china (nsf) (2005-2007, and 2006-2008, respectively).
recent publications
? liu b, li z, hong y, ni j, sheng d, shen y .( 2006) cloning, expression and characterization of a thermostable exo-beta-d-glucosaminidase from the hyperthermophilic archaeon pyrococcus horikoshii . biotechnol lett (in press).
? liu,b., ni j., shen y . (2006) cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeion pyrococcus horikoshii . acta microbiologica sinica 46:255-258.
? tang, x.-f, shen y. , matsui e., matsui i (2004) domain topology of the dna polymerase d complex from a hyperthermophilic archaeon pyrococcus horikoshii . biochemistry. 43:11818-27.
? shen, y. , tang x.-f., matsui e., matsui i. (2004). subunit interaction and regulation of activity through terminal domains of the family d dna polymerse from pyrococcus horikoshii. biochem. soc. trans. 32:245-9.
? shen, y. , tang x.-f., yokoyama h., matsui e., and matsui i. (2004) a 21-aa peptide from the cysteine cluster ii of the family d dna polymerase from pyrococcus horikoshii stimulates its nuclease activity which is mre11-like and prefers manganese ion as the co-factor. nucl. acids res. 32: 158-68.
? shen,y ., tang x .-f ., and matsui i. (2003) subunit interaction and regulation of activity through terminal domains of the family d dna polymerase from pyrococcus horikoshii . j. biol . chem . 278 :21247-57.
? shen, y. , musti k., hiramoto m., kikuchi h., kawabayashi y. and matsui, i. (2001) invariant asp1122 and asp1124 are essential residues for polymerization catalysis of family d dna polymerase from pyrococcus horikoshii . j. biol. chem. 276: 27376-83.
? zheng r., matsui e., shen y. , kishnasastry m., feng y., darnis s., kawarabayasi y., kikuchi h., harata k., and matsui i. (2001) the novel function of a short region k 253 xrxxxd 259 conserved in the exonuclease domain of hyperthermostable dna polymerase i from pyrococcus horikoshii . extremophiles 5: 111-7.
? shen, y ., newbury hj and ford-lloyd bv (1998) identification of taxa in the genus beta using its1 sequence information. plant mol. biol. rep. 16: 147-55.
? shen, y. , ford-lloyd bv and newbury hj (1998) genetic relationship using both pcr-based and dna sequencing methods. heredity 80: 624-32.
? shen, y ., newbury hj and ford-lloyd bv (1996) the taxonomic characterization of annual beta germplasm in a genetic resources collection using rapd makers. euphytica 91: 205-12.
申玉龙
博士 , 教授 , 博士生导师
tel. 0531-88362928 e-mail: yulgshen@sdu.edu.cn
主要履历与经历
1982-1986 年 兰州大学 学士
1986-1989 年 兰州大学 硕士
? 中国农业科学院兰州畜牧研究所 实习研究员
1993-1996 年 英国伯明翰大学 博士
1997-1999 年 日本农林水产省果树研究所 博士后
1999-2001 年 日本通产省工业技术院生命工学研究所 研究员
2001-2003 年 日本产业技术综合研究所生物信息研究中心 研究员
2003 年 - 现在 山东大学微生物技术国家重点实验室 教授
主要研究领域与承担项目
古菌 dna 复制、修复和重组的分子机制，极端环境微生物资源与极端酶的研究与利用。目前承担国家重点基础研究发展（ 973 ）计划子课题“ 极端嗜 热 古菌 dna 修 复 与重 组 机制” 一项 (2004 - 2008) 。国家自然科学基金面上项目：“极端嗜热古菌 dna 复制、修复和重组酶的研究” (2005 - 2007) 和 “超嗜热古菌特有几丁质酶系研究” (2006 - 2008) 两项。
recent publications
? liu b, li z, hong y, ni j, sheng d, shen y .( 2006) cloning, expression and characterization of a thermostable exo-beta-d-glucosaminidase from the hyperthermophilic archaeon pyrococcus horikoshii . biotechnol lett (in press).
? liu,b., ni j., shen y . (2006) cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeion pyrococcus horikoshii . acta microbiologica sinica 46:255-258.
? tang, x.-f, shen y. , matsui e., matsui i (2004) domain topology of the dna polymerase d complex from a hyperthermophilic archaeon pyrococcus horikoshii . biochemistry. 43:11818-27.
? shen, y. , tang x.-f., matsui e., matsui i. (2004). subunit interaction and regulation of activity through terminal domains of the family d dna polymerse from pyrococcus horikoshii. biochem. soc. trans. 32:245-9.
? shen, y. , tang x.-f., yokoyama h., matsui e., and matsui i. (2004) a 21-aa peptide from the cysteine cluster ii of the family d dna polymerase from pyrococcus horikoshii stimulates its nuclease activity which is mre11-like and prefers manganese ion as the co-factor. nucl. acids res. 32: 158-68.
? shen,y ., tang x .-f ., and matsui i. (2003) subunit interaction and regulation of activity through terminal domains of the family d dna polymerase from pyrococcus horikoshii . j. biol . chem . 278 :21247-57.
? shen, y. , musti k., hiramoto m., kikuchi h., kawabayashi y. and matsui, i. (2001) invariant asp1122 and asp1124 are essential residues for polymerization catalysis of family d dna polymerase from pyrococcus horikoshii . j. biol. chem. 276: 27376-83.
? zheng r., matsui e., shen y. , kishnasastry m., feng y., darnis s., kawarabayasi y., kikuchi h., harata k., and matsui i. (2001) the novel function of a short region k 253 xrxxxd 259 conserved in the exonuclease domain of hyperthermostable dna polymerase i from pyrococcus horikoshii . extremophiles 5: 111-7.
? shen, y ., newbury hj and ford-lloyd bv (1998) identification of taxa in the genus beta using its1 sequence information. plant mol. biol. rep. 16: 147-55.
? shen, y. , ford-lloyd bv and newbury hj (1998) genetic relationship using both pcr-based and dna sequencing methods. heredity 80: 624-32.
? shen, y ., newbury hj and ford-lloyd bv (1996) the taxonomic characterization of annual beta germplasm in a genetic resources collection using rapd makers. euphytica 91: 205-12.
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